Premium
Detection of carbapenem resistance genes and cephalosporin, and quinolone resistance genes along with oqxAB gene in E scherichia coli in hospital wastewater: a matter of concern
Author(s) -
Chandran S.P.,
Diwan V.,
Tamhankar A.J.,
Joseph B.V.,
RosalesKlintz S.,
Mundayoor S.,
Lundborg C.S.,
Macaden R.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12591
Subject(s) - cephalosporin , microbiology and biotechnology , imipenem , escherichia coli , quinolone , biology , plasmid , carbapenem , multiple drug resistance , drug resistance , ciprofloxacin , gene , antibiotic resistance , antibiotics , genetics
Aims This study was performed to detect the presence of E scherichia coli resistant to cephalosporins, carbapenems and quinolones in hospital wastewater. Methods and Results Wastewaters from a rural (H1) and an urban (H2) hospital were tested for E. coli resistant to cephalosporins, carbapenem and quinolones. Genes coding for chromosomal and plasmid‐mediated resistance and phylogenetic grouping was detected by multiplex polymerase chain reaction ( PCR ) and for genetic relatedness by rep‐ PCR . Of 190 (H1 = 94; H2 = 96) E. coli examined, 44% were resistant to both cephalosporins and quinolones and 3% to imipenem. ESBL s were detected phenotypically in 96% of the isolates, the gene bla CTX ‐M coding for 87% and bla TEM for 63%. Quinolone resistance was due to mutations in gyr A and par C genes in 97% and plasmid‐coded aac‐ (6′) ‐Ib‐cr in 89% of isolates. Only in one carbapenem‐resistant E. coli, NDM ‐1 was detected. Nearly 67% of the isolates belonged to phylogenetic group B2. There was no genetic relatedness among the isolates. Conclusions Hospital wastewater contains genetically diverse multidrug‐resistant E. coli . Significance and Impact of the Study This study stresses the need for efficient water treatment plants in healthcare settings as a public health measure to minimize spread of multidrug‐resistant bacteria into the environment.