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Association of multicellular behaviour and drug resistance in S almonella enterica serovars isolated from animals and humans in E thiopia
Author(s) -
Eguale T.,
Marshall J.,
Molla B.,
Bhatiya A.,
Gebreyes W.A.,
Engidawork E.,
Asrat D.,
Gunn J.S.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12579
Subject(s) - salmonella enterica , biology , salmonella , integron , microbiology and biotechnology , multicellular organism , serotype , multiple drug resistance , biofilm , antibiotic resistance , genomic island , drug resistance , bacteria , genetics , antibiotics , plasmid , gene
Aims To determine the association between multicellular behaviour, integron status and antibiotic resistance among 87 Ethiopian S almonella enterica isolates of animal and human origin. Methods and Results Isolates were characterized for their biofilm forming ability, antimicrobial susceptibility and the presence and characteristics of a class 1 integron and S almonella genomic island 1 ( SGI 1). The majority of isolates grown at environmental temperatures (20°C) exhibited robust biofilm formation (72·4%) and displayed RDAR colony morphology on Congo red agar plates. The presence of a class 1 integron correlated with the extent of drug resistance and ability to exhibit multicellular behaviour. Conclusions Although cellulose production and RDAR morphology correlated with increased multicellular behaviour, neither was required for biofilm formation. Contrary to previous reports, colony morphology was generally consistent within a serovar. No integrons were detected in isolates deficient for multicellular behaviour, indicating a potential role of bacterial community formation in transfer of genetic elements among environmental isolates. Significance and Impact of Study Infection by S alm. enterica is a major public health problem worldwide. The dominance of multidrug resistance and multicellular behaviour in S almonella isolates of Ethiopian origin highlights a need for integrated surveillance and further detailed phenotypic and molecular studies of isolates from this region.