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Multilocus sequence analysis of ‘ C andidatus P hytoplasma asteris’ strain and the genome analysis of T urnip mosaic virus co‐infecting oilseed rape
Author(s) -
Šeruga Musić M.,
Duc Nguyen H.,
Černi S.,
Mamula Đ.,
Ohshima K.,
Škorić D.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12567
Subject(s) - biology , turnip mosaic virus , phyllody , phytoplasma , phylogenetic tree , gene , genetics , genome , sequence analysis , whole genome sequencing , virology , phylogenetics , oryx , restriction fragment length polymorphism , virus , polymerase chain reaction , potyvirus , plant virus , paleontology
Aim Molecular characterization of a pathogenic complex infecting winter oilseed rape ( Brassica napus ssp. oleifera ( DC .) Metzg.) plants showing typical rape phyllody symptoms along with some atypical changes. Methods and Results Phytoplasma (‘ Candidatus Phytoplasma’) presence was confirmed by PCR‐RFLP and 16S r RNA gene sequencing. Phylogenetic analyses of phytoplasma amp , tufB , secY , groEL and ribosomal protein genes confirmed its affiliation to the ‘ Ca . P. asteris’ species. However, in the amp gene encoding a specific protein crucial for insect transmission specificity, significant SNPs were found. Biological and serological tests revealed the co‐infection with Turnip mosaic virus (TuMV). The phylogenetic analysis of full TuMV genome sequence, the first reported from the Balkans, classified it into the world‐B phylogenetic lineage. Conclusions A pathogenic complex consisting of ‘ Ca . P. asteris’ and Tu MV found to co‐infect oilseed rape plants for the first time was molecularly characterized. Significance and Impact of the Study Rape phyllody is a serious problem in rapeseed production. The molecular information from this first multi‐gene analysis of ‘ Ca . P. asteris’ strain associated with rape phyllody as well as the first report of the complete sequence of Tu MV isolate from the Balkans is a starting point for understanding the disease complexity and management.