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Immunoproteomic analysis of antibody response to cell wall‐associated proteins of C andida tropicalis
Author(s) -
Lee P.Y.,
Gam L.H.,
Yong V.C.,
Rosli R.,
Ng K.P.,
Chong P.P.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12562
Subject(s) - candida tropicalis , antigen , biology , microbiology and biotechnology , immune system , western blot , candida albicans , candida parapsilosis , antibody , escherichia coli , immunology , gene , genetics
Aims This study was conducted to identify antigenic proteins of C andida tropicalis that are targeted by the host immune system. Methods and Results An immunoproteomic approach was used to discover antigens from cell wall of C . tropicalis that were recognized by sera from experimentally infected mice. This resulted in the identification of twelve distinct proteins, of which ten have been previously reported as antigens of C andida albicans . For the remaining two proteins, Idh2p has been described as an antigen of C andida parapsilosis, whereas Kgd2p is revealed for the first time as an antigenic protein for Candida species. These two antigens were expressed as recombinant proteins in E scherichia coli and were shown to be specifically recognized by sera from infected host on Western blot. Conclusions The present work investigated immunoproteome of C . tropicalis and identified several biomarker candidate antigens, with Kgd2p as a novel immunogenic protein that could be associated with pathogenesis of C . tropicalis . Significance and Impact of the Study Findings from this study help to improve current understanding on host response to C . tropicalis infection and provide new insights into immune‐pathogenesis of C . tropicalis . Besides, the immunogenic proteins could be considered as targets for the development of immunodiagnostic assay and/or vaccine.