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Effect of nanosecond pulsed electric field on E scherichia coli in water: inactivation and impact on protein changes
Author(s) -
Guionet A.,
JoubertDurigneux V.,
Packan D.,
Cheype C.,
Garnier J.P.,
David F.,
Zaepffel C.,
Leroux R.M.,
Teissié J.,
Blanckaert V.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12558
Subject(s) - escherichia coli , propidium iodide , bacteria , nanosecond , microbiology and biotechnology , chemistry , bacterial outer membrane , biology , biophysics , food science , biochemistry , genetics , apoptosis , laser , physics , programmed cell death , optics , gene
Aims This article shows the effect of nanosecond pulsed electric field (ns PEF ) on E scherichia coli , which could imply a durable change in protein expressions and then impacted the phenotype of surviving bacteria that might lead to increase pathogenicity. Methods and Results The effects of ns PEF on E . coli viability and membrane permeabilization were investigated. One log 10 reduction in bacterial counts was achieved at field strength of 10 7 V m −1 with a train of 500 successive pulses of 60 × 10 −9 s. Incubation of germs after treatment with propidium iodide showed that membrane permeabilization was reversible. Possible protein changes of surviving bacteria were checked to assess potential phenotypical changes using two‐dimensional electrophoresis. In our study, after 40 generations, only UniProt #P39187 was up‐regulated with P ≤ 0·05 compared with the control and corresponded to the uncharacterized protein YtfJ. Antibiograms were used to check whether or not the pattern of cultivable bacteria after ns PEF deliveries changed. Conclusions The results tend to show that ns PEF s are able to inactivate bacteria and have probably no serious impact in E . coli protein patterns. Significance and Impact of the Study The use of ns PEF is a safe promising new nonthermal method for bacterial inactivation in the food processing and environmental industry.