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Matrine reduces yeast‐to‐hypha transition and resistance of a fluconazole‐resistant strain of C andida albicans
Author(s) -
Shao J.,
Wang T.,
Yan Y.,
Shi G.,
Cheng H.,
Wu D.,
Wang C.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12555
Subject(s) - hypha , matrine , candida albicans , microbiology and biotechnology , fluconazole , corpus albicans , yeast , minimum inhibitory concentration , biology , agar , biofilm , chemistry , antimicrobial , antifungal , bacteria , biochemistry , genetics , neuroscience
Aims To evaluate the potential effect of matrine on reducing the growth of hypha and lowering the resistance of a fluconazole‐resistant colony of Candida albicans . Methods and Results Candida albicans SC 5314 and a fluconazole‐resistant C. albicans 215 were used. As for C. albicans SC 5314, minimal inhibitory concentration ( MIC 80 ) and effective concentration ( EC 50 ) were determined, 1 mg ml −1 matrine could inhibit nearly 80% of planktonic growth by inverted microscope, 2 mg ml −1 matrine suppressed 50% of metabolic activity of biofilm by XTT assay, vanishing hypha could be observed on spider agar containing 2 mg ml −1 matrine, the expressions of three hypha‐related genes, namely ALS 3 , SUN 41 and PBS 2 , were suppressed by 29, 45 and 61% by 2 mg ml −1 matrine. Also, matrine could lower the resistance of C. albicans 215, in either the free‐floating form or the biofilm phenotype. Conclusions Matrine had favourable antifungal potential and might be able to reverse the fluconazole resistance of clinical isolates at relatively high concentration. The anti‐ candidal performance of matrine could be tightly associated with yeast‐to‐hypha transition proved by spider agar test and qRT ‐PCR. Significance and Impact of Study More efforts are needed to find new antifungal agents. Matrine could be a potential candidate to fight against Candida ‐related infections by regulating yeast‐to‐hypha transition.