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Characterization of E scherichia fergusonii and E scherichia albertii isolated from water
Author(s) -
Maheux A.F.,
Boudreau D.K.,
Bergeron M.G.,
Rodriguez M.J.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12551
Subject(s) - escherichia coli , biology , housekeeping gene , microbiology and biotechnology , multilocus sequence typing , phylogenetic tree , gene , genetics , genotype , gene expression
Abstract Aims The aim of this study was to characterize E scherichia fergusonii and E scherichia albertii isolated from water. Methods and Results The characterization of E . fergusonii and E . albertii isolated from water was determined using an E scherichia coli ‐specific uid A PCR , a tuf PCR , and with phylogenetic analysis using three housekeeping genes ( adk , gyr B , and rec A ) from the E . coli MLST scheme, selected for their ability to discriminate among all E scherichia species. Among the 527 isolates tested, 25 (4·7%) were uidA PCR negative and tuf PCR positive. Phylogenetic analysis using adk , gyr B and rec A genes showed that 6, 18 and 1 of these 25 non‐ E . coli E scherichia spp. isolates grouped with reference strains of E . fergusonii , E . albertii , and E . coli , respectively. Finally, the 25 non‐ E . coli E scherichia spp. strains isolated were investigated for the presence of pathogenic factors, comprising intimin ( eae gene), cytolethal distending toxin ( cdt B gene) and shiga toxin ( stx gene). With the PCR primers used, the presence of eae and stx genes was not detected. However, cdt B genes types I/ IV were detected for 3 (16·7%) E . albertii strains, whereas 15 of 18 (83·3%) possessed the cdt B gene types II / III /V. Conclusions These results showed that MLST scheme allows a more accurate identification of non‐ E. coli species than phenotypic tests. We also showed that E . fergusonii and E . albertii represent, respectively, 0·8 and 2·5% of all E scherichia species isolated and the pathogenic cdtB genes were present in 83·3% of these strains. Significance and impact of the study The data presented in this study provided an efficient way to correctly identify non‐ E . coli species contributing to our understanding of the risks associated with E scherichia species in water consumed by humans and animals. Furthermore, the results give an insight about the natural habitats of these species.