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In vitro evaluation of the mucin‐adhesion ability and probiotic potential of L actobacillus mucosae LM 1
Author(s) -
Valeriano V.D.,
ParungaoBalolong M.M.,
Kang D.K.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12539
Subject(s) - probiotic , library science , medicine , biology , computer science , bacteria , genetics
Aims In this report, we characterized the probiotic potential of L actobacillus mucosae LM 1, focusing on its in vitro mucin‐adhesion abilities. Methods and Results Screening assays were used to evaluate LM 1. Previous studies on L act. mucosae species have been performed, but few have examined the ability of this species to adhere to and colonize the intestinal mucosa. Thus, adhesion, aggregation and pathogen inhibition assays of LM 1 along with microbial adhesion to solvents ( MATS ) assay were carried out in comparison with another putative probiotic, L actobacillus johnsonii PF 01, and the commercial strain, L actobacillus rhamnosus GG . Based on MATS assay, the cell surfaces of the lactobacilli strains were found to be hydrophobic and highly electron‐donating, but the average hydropathy ( GRAVY ) index of predicted surface‐exposed proteins in the LM 1 genome indicated that most were hydrophilic. LM 1 showed the highest adhesion, aggregation and hydrophobicity among the strains tested and significantly inhibited the adhesion of E scherichia coli K88 and S almonella enterica serovar T yphimurium KCCM 40253. Correlations among adhesion, aggregation and hydrophobicity, as well as between coaggregation and displacement of E . coli , were observed. Conclusions Increased adhesion may not always correlate with increased pathogen inhibition due to various strain‐specific mechanisms. Nevertheless, LM 1 has promising probiotic properties that can be explored further using a genomics approach. Significance and Impact of the Study Our data on adhesion of LM 1 strain showed a significant correlation between adhesion, hydrophobicity of cell surface and autoaggregation. This study gives basic knowledge for the elucidation of the adhesion mechanism of Lactobacillus sp. and prediction of its adherence in specific host models.