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Inhibition of S almonella typhi growth using extremely low frequency electromagnetic ( ELF ‐ EM ) waves at resonance frequency
Author(s) -
Fadel M.A.,
Mohamed S.A.,
Abdelbacki A.M.,
ElSharkawy A.H.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12527
Subject(s) - salmonella typhi , typhoid fever , salmonella , resonance (particle physics) , in vivo , extremely low frequency , in vitro , electromagnetic radiation , microbiology and biotechnology , biology , electromagnetic field , biophysics , chemistry , physics , bacteria , optics , biochemistry , escherichia coli , genetics , particle physics , quantum mechanics , gene
Aims Typhoid is a serious disease difficult to be treated with conventional drugs. The aim of this study was to demonstrate a new method for the control of Salmonella typhi growth, through the interference with the bioelectric signals generated from the microbe during cell division by extremely low frequency electromagnetic waves ( ELF ‐ EMW ‐ ELF ‐ EM ) at resonance frequency. Methods and Results Isolated Salmonella typhi was subjected to square amplitude modulated waves ( QAMW ) with different modulation frequencies from two generators with constant carrier frequency of 10 MHz, amplitude of 10 Vpp, modulating depth ± 2 Vpp and constant field strength of 200 V m −1 at 37°C. Both the control and exposed samples were incubated at the same conditions during the experiment. The results showed that there was highly significant inhibition effect for Salm. typhi exposed to 0·8 Hz QAMW for a single exposure for 75 min. Dielectric relaxation, TEM and DNA results indicated highly significant changes in the molecular structure of the DNA and cellular membrane resulting from the exposure to the inhibiting EM waves. Conclusions It was concluded that finding out the inhibiting resonance frequency of ELF ‐ EM waves that deteriorates Salm. typhi growth will be promising method for the treatment of Salm. typhi infection either in vivo or in vitro . Significance and Impact of the Study This new non‐invasive technique for treatment of bacterial infections is of considerable interest for the use in medical and biotechnological applications.