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Investigation into in vitro and in vivo models using intestinal epithelial IPEC ‐J2 cells and C aenorhabditis elegans for selecting probiotic candidates to control porcine enterotoxigenic E scherichia coli
Author(s) -
Zhou M.,
Zhu J.,
Yu H.,
Yin X.,
Sabour P.M.,
Zhao L.,
Chen W.,
Gong J.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12505
Subject(s) - caenorhabditis elegans , probiotic , biology , lactobacillus reuteri , microbiology and biotechnology , enterotoxigenic escherichia coli , enterotoxin , antimicrobial , lactobacillus , in vivo , escherichia coli , in vitro , gene , bacteria , genetics
Aims To identify a fast, economic and reliable method for preselecting lactic acid‐producing bacterial ( LAB ) isolates to control enterotoxigenic E scherichia coli ( ETEC ). Methods and Results Two assays with porcine intestinal epithelial IPEC ‐J2 cells or C aenorhabditis elegans for selecting effective probiotic candidates were compared. Both assays were based on measuring death of cells or worms caused by ETEC strain JG 280. Six of 13 LAB isolates showed ≥50% protection in each assay, among which only four isolates (≥50% protection) were consistently selected by both assays. Isolate CL 9 ( L actobacillus reuteri ) was further studied. It reduced gene expression of estA , estB and elt in JG 280 in both assays. Furthermore, the isolate protected IPEC ‐J2 and C. elegans from cell and worm death caused by ST a, ST b or LT enterotoxin expressed in E. coli DH 5 α . CL 9 also promoted host defensive responses by decreasing IL ‐8 and increasing IL ‐10 production in IPEC ‐J2 cells and expression of antimicrobial peptide genes clec‐60 , clec‐85 in C. elegans . Conclusions Caenorhabditis elegans is useful for preselecting probiotic candidates to control ETEC after initial screening with IPEC ‐J2 cells. Significance and Impact of the Study A combination of IPEC ‐J2 cell and C. elegans assays can improve the effectiveness in preselecting probiotic candidates.