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A combination of MLST and CRISPR typing reveals dominant Campylobacter jejuni types in organically farmed laying hens
Author(s) -
Kovanen S.M.,
Kivistö R.I.,
Rossi M.,
Hänninen M.L.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12503
Subject(s) - multilocus sequence typing , biology , crispr , typing , campylobacter jejuni , campylobacter , microbiology and biotechnology , veterinary medicine , genetics , gene , genotype , bacteria , medicine
Aim To elucidate the Campylobacter jejuni population in organically farmed laying hens in Finland, multilocus sequence typing ( MLST ) was combined with characterization of clustered regularly interspaced short palindromic repeat ( CRISPR ) sequences. Methods and Results A total of 147 Camp. jejuni isolates, collected from organically farmed laying hens from 18 farms in 2003–2004, were previously analysed by pulsed‐field gel electrophoresis. In the present study, subsets of the isolates were further analysed by MLST and CRISPR sequences. Fourteen ST s were found by MLST . ST ‐50 (27%, 7/18 farms), ST ‐3272 (20%, 8/18 farms), ST ‐45 (12%, 7/18 farms) and ST ‐356 (12%, 5/18 farms) were the most common ST s. CRISPR types were identical among all isolates of ST ‐50 ( ST ‐21 clonal complex ( CC )) and the most variable among ST ‐45 ( ST ‐45 CC ). Conclusions ST ‐3272 ( UA ), a common ST in this study, has been infrequently detected in other hosts. Other major ST s ( ST ‐50 and ST ‐45) have been common in several hosts such as conventional poultry and bovines. CRISPR typing provided additional discrimination between isolates of certain dominant ST s and could be useful in further epidemiological studies. Significance and Impact of the Study This study gives new information about MLST and CRISPR types of C amp. jejuni among organically farmed laying hens.