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A novel echinocandin MIG 0310 with anticandida activity from newly isolated F usarium sp. strain MS ‐R1
Author(s) -
Masaphy S.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12493
Subject(s) - mycology , clinical microbiology , fusarium , strain (injury) , microbiology and biotechnology , echinocandin , library science , medicine , biology , antifungal , botany , computer science , fluconazole
Aim To isolate and identify an anticandida compound from newly isolated F usarium strain MS ‐ R 1 and characterization of its activity against standard and clinical strains of C andida. Methods and Results The fungal strain, F usarium strain MS ‐ R 1, was isolated from soil. Molecular identification according to the internal transcribed spacer region of the rRNA gene sequence showed the strain to be strongly related to F usarium brachygibbosum complex. Successive extractions of the active compound showed activity against C andida albicans , including clinical strains. Inhibition of a clinical strain of C andida tropicalis , but not C andida krusei and C andida glabrata , was also shown as tested by the broth microdilution assay. The compound was purified by liquid chromatography coupled with thin‐layer chromatography and bioautography and characterized by nuclear magnetic resonance spectroscopy. It was confirmed to have the molecular formula C 48 H 66 O 18 and was identified as an echinocandin with a novel structure. Conclusion A novel echinocandin‐type antifungal metabolite, MIG 0310, was isolated and characterized. This is a second echinocandin reported from a F usarium species, indicating this genus to have wider range of echinocandin compounds. Significance and Impact of the Study The new compound may lead to new anticandidal drugs, broadening the treatment opportunities for C andida species, including those that are resistant to the current antifungal drugs.