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Effects of extracellular DNA from C andida albicans and pneumonia‐related pathogens on C andida biofilm formation and hyphal transformation
Author(s) -
Sapaar B.,
Nur A.,
Hirota K.,
Yumoto H.,
Murakami K.,
Amoh T.,
Matsuo T.,
Ichikawa T.,
Miyake Y.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12483
Subject(s) - biofilm , microbiology and biotechnology , candida albicans , hypha , biology , candida tropicalis , candida glabrata , corpus albicans , yeast , pseudomonas aeruginosa , bacteria , biochemistry , genetics
Aims The aim of this study was to investigate the effects of genomic DNA purified from C andida albicans and pneumonia‐related pathogens, Pseudomonas aeruginosa and Staphylococcus aureus , on in vitro biofilm formation and morphological change of 3 Candida species ( C . albicans , C . glabrata , and C . tropicalis ). Methods and Results Biofilm formation was evaluated by the crystal violet assay and colony‐forming unit counts. Morphological characteristics of biofilms were evaluated by scanning electron microscopy and fluorescence microscopy. Addition of DNA at a low concentration (<1·0 μ g ml −1 ) significantly increased biofilm mass of all three Candida species. In contrast, the addition of DNA at a high concentration (10 μ g ml −1 ) decreased the biofilm mass. Interestingly, the formation of hyphae in a dense network of yeast cells was observed in C . albicans biofilms exposed to a low concentration of DNA (<1·0 μ g ml −1 ). Conclusions These findings demonstrated that extracellular DNA ( eDNA ) plays a crucial role in C andida biofilm formation and suggested that eDNA may induce the morphological transition from yeast to hyphal growth form during C . albicans biofilm development. Significance and Impact of the Study A novel therapy targeting eDNA may be applicable for C andida infection to decrease biofilm formation and hyphal formation.