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Superoxide dismutase recombinant L actobacillus fermentum ameliorates intestinal oxidative stress through inhibiting NF‐ κ B activation in a trinitrobenzene sulphonic acid‐induced colitis mouse model
Author(s) -
Hou C.L.,
Zhang J.,
Liu X.T.,
Liu H.,
Zeng X.F.,
Qiao S.Y.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12461
Subject(s) - lactobacillus fermentum , superoxide dismutase , recombinant dna , colitis , oxidative stress , myeloperoxidase , inflammatory bowel disease , chemistry , inflammation , biology , immunology , lactic acid , biochemistry , medicine , bacteria , genetics , disease , gene , lactobacillus plantarum
Aims Superoxide dismutase ( SOD ) can prevent and cure inflammatory bowel diseases by decreasing the amount of reactive oxygen species. Unfortunately, short half‐life of SOD in the gastrointestinal tract limited its application in the intestinal tract. This study aimed to investigate the treatment effects of recombinant SOD Lactobacillus fermentum in a colitis mouse model. Methods and Results In this study, we expressed the sodA gene in L act. fermentum I 5007 to obtain the SOD recombinant strain. Then, we determined the therapeutic effects of this SOD recombinant strain in a trinitrobenzene sulphonic acid ( TNBS ) ‐ induced colitis mouse model. We found that SOD activity in the recombinant L act. fermentum was increased by almost eightfold compared with that in the wild type. Additionally, both the wild type and the recombinant L act. fermentum increased the numbers of lactobacilli in the colon of mice ( P < 0·05). Colitis mice treated with recombinant L act. fermentum showed a higher survival rate and lower disease activity index ( P < 0·05). Recombinant L act. fermentum significantly decreased colonic mucosa histological scoring for infiltration of inflammatory cells, lipid peroxidation, the expression of pro ‐ inflammatory cytokines and myeloperoxidase ( P < 0·05) and inhibited NF ‐ κ B activity in colitis mice ( P < 0·05). Conclusions SOD recombinant L act. fermentum significantly reduced oxidative stress and inflammation through inhibiting NF ‐ κ B activation in the TNBS ‐induced colitis model. Significance and Impact of the Study This study provides insights into the anti‐inflammatory effects of SOD recombinant L act. fermentum , indicating the potential therapeutic effects in preventing and curing intestinal bowel diseases.