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Nonthermal plasma affects viability and morphology of M ycoplasma hominis and A choleplasma laidlawii
Author(s) -
Ermolaeva S.A.,
Rakovskaya I.V.,
Miller G.G.,
Sysolyatina E.V.,
Mukhachev A.Y.,
Vasiliev M.M.,
Adgamov R.R.,
Levina G.A.,
Petrov O.F.,
Morfill G.E.,
Grigoriev A.I.,
Fortov V.E.,
Gintsburg A.L.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12445
Subject(s) - mycoplasma hominis , mollicutes , microbiology and biotechnology , butylated hydroxytoluene , bacteria , biology , reactive oxygen species , staphylococcus aureus , mycoplasma , chemistry , antioxidant , biochemistry , genetics
Abstract Aim To study the effects exerted by argon microwave nonthermal plasma ( NTP ) on cell wall‐lacking M ollicutes bacteria. Methods and Results 10 8 CFU ml −1 agar plated M ycoplasma hominis and A choleplasma laidlawii were treated with the nonthermal microwave argon plasma for 30–300 s. The maximal 10‐ and 100‐fold drop was observed for A . laidlawii and M yc. hominis , respectively. Similarly treated E scherichia coli and S taphylococcus aureus demonstrated the 10 5 and 10 3 drop, respectively. Removal of cholesterol affected resistance of A . laidlawii . 10 mmol l −1 antioxidant butylated hydroxytoluene decreased mortality by a factor of 25–200. UV radiation alone caused 25–85% mortality in comparison with the whole NTP. Exogenously added hydrogen peroxide H 2 O 2 did not cause mortality. NTP treatment of Myc. hominis triggered growth of microcolonies, which were several tenfold smaller than a typical colony. Conclusions Despite the lack of cell wall, A. laidlawii and Myc. hominis were more resistant to argon microwave NTP than other tested bacteria. M ycoplasma hominis formed microcolonies upon NTP treatment. A role of UV and active species was demonstrated. Significance and Impact of the Study The first study of NTP effects on M ollicutes revealed importance of a membrane composition for bacterial resistance to NTP . New specific M yc. hominis morphological forms were observed. The study confirmed importance of the concerted action of reactive oxygen species (ROS) with UV and other plasma bioactive agents for NTP bactericidal action.