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L actobacillus gasseri K 7 modulates the blood cell transcriptome of conventional mice infected with E scherichia coli O 157: H 7
Author(s) -
Sagaya F.M.,
Hacin B.,
Tompa G.,
Ihan A.,
Špela Š.,
Černe M.,
Hurrell R.F.,
Matijašić B.B.,
Rogelj I.,
Vergères G.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12440
Subject(s) - lactobacillus gasseri , transcriptome , escherichia coli , biology , microbiology and biotechnology , lactobacillus , bacteria , gene , gene expression , genetics
Aims As the immune cells underlying the intestinal barrier sense luminal microbial signals, blood cell transcriptomics may identify subclinical changes triggered by gut bacteria that may otherwise not be detected. We have therefore investigated how L actobacillus gasseri K 7 and enterohemorrhagic E scherichia coli O 157: H 7 modulate the blood cell transcriptome of mice possessing an intact microbiota. Methods and Results We have analysed the transcriptome of five groups of C 57 BL /6J mice: (i) control, (ii) inoculated with a single dose of E . coli , (iii) inoculated during 2 weeks with L act . gasseri , (iv) co‐inoculated with E . coli and L act. gasseri , (v) inoculated with L act. gasseri prior to E . coli infection. The transcriptome could distinguish between the five treatment groups. Gene characteristics of bacterial infection, in particular inflammation, were upregulated in the mice inoculated with E. coli . L act. gasseri had only mild effects on the transcriptome but modified the gene expression induced by E . coli . Conclusions The transcriptome differentiates mice inoculated orally with E . coli, L act. gasseri and combinations of these two strains. Significance and Impact of the Study These results suggest that the blood cell transcriptome can be used as a source of biomarkers to monitor the impact of probiotics in subclinical models of infectious disease.