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Rapid identification of E nterobacter hormaechei and E nterobacter cloacae genetic cluster III
Author(s) -
Ohad S.,
Block C.,
Kravitz V.,
Farber A.,
Pilo S.,
Breuer R.,
Rorman E.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12439
Subject(s) - tel aviv , christian ministry , public health , library science , sociology , political science , medicine , law , computer science , nursing
Aim E nterobacter cloacae complex bacteria are of both clinical and environmental importance. Phenotypic methods are unable to distinguish between some of the species in this complex, which often renders their identification incomplete. The goal of this study was to develop molecular assays to identify E nterobacter hormaechei and E nt. cloacae genetic cluster III which are relatively frequently encountered in clinical material. Methods and Results The molecular assays developed in this study are q PCR technology based and served to identify both E nt. hormaechei and E nt. cloacae genetic cluster III . q PCR results were compared to hsp60 sequence analysis. Most clinical isolates were assigned to E nt. hormaechei subsp. steigerwaltii and E nt. cloacae genetic cluster III . The latter was proportionately more frequently isolated from bloodstream infections than from other material ( P < 0·05). Conclusion The q PCR assays detecting E nt. hormaechei and E nt. cloacae genetic cluster III demonstrated high sensitivity and specificity. Significance and Impact of the Study The presented q PCR assays allow accurate and rapid identification of clinical isolates of the E nt. cloacae complex. The improved identifications obtained can specifically assist analysis of E nt. hormaechei and E nt. cloacae genetic cluster III in nosocomial outbreaks and can promote rapid environmental monitoring. An association was observed between E nt. cloacae cluster III and systemic infection that deserves further attention.