Multicenter evaluation of the  S epsityper™ extraction kit and  MALDI ‐ TOF MS  for direct identification of positive blood culture isolates using the  BD BACTEC ™  FX  and Versa TREK  ®  diagnostic blood culture systems | Zendy

Schieffer K.M. | Zendy; Tan K.E. | Zendy; Stamper P.D. | Zendy; Somogyi A. | Zendy; Andrea S.B. | Zendy; Wakefield T. | Zendy; Romagnoli M. | Zendy; Chapin K.C. | Zendy; Wolk D.M. | Zendy; Carroll K.C. | Zendy

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Multicenter evaluation of the S epsityper™ extraction kit and MALDI ‐ TOF MS for direct identification of positive blood culture isolates using the BD BACTEC ™ FX and Versa TREK ® diagnostic blood culture systems

Author(s) -

Schieffer K.M.,

Tan K.E.,

Stamper P.D.,

Somogyi A.,

Andrea S.B.,

Wakefield T.,

Romagnoli M.,

Chapin K.C.,

Wolk D.M.,

Carroll K.C.

Publication year - 2014

Publication title -

journal of applied microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.889

H-Index - 156

eISSN - 1365-2672

pISSN - 1364-5072

DOI - 10.1111/jam.12434

Subject(s) - chromatography , extraction (chemistry) , blood culture , sample preparation , matrix assisted laser desorption/ionization , mass spectrometry , chemistry , biochemistry , antibiotics , organic chemistry , adsorption , desorption

Aims (i) Evaluation of delayed time to blood culture extraction by the S epsityper kit and impact of shipping pellets off‐site for MALDI ‐ TOF MS analysis. (ii) Comparison of S epsityper and laboratory‐developed extraction methods from a literature review. Methods and Results Using two blood culture systems ( BD BACTEC and Versa TREK ), we extracted 411 positive blood cultures using the S epsityper kit to mimic a potential protocol for institutions without a MALDI ‐ TOF MS . Extracted pellets were shipped and analysed on the B ruker U ltraflex III . Successful extraction of 358 (87·1%) samples was determined by the presence of detectable proteins. MALDI ‐ TOF MS correctly identified 332 (80·8%) samples. Conclusions Delayed time to extraction did not affect S epsityper extraction or MALDI ‐ TOF MS accuracy. The extracted pellets remain stable and provide accurate results by MALDI ‐ TOF MS when shipped at room temperature to off‐site reference laboratories. Significance and Impact of the Study This is the first study to show that institutions without a MALDI ‐ TOF MS can take advantage of this innovative technology by shipping a volume of blood to an off‐site laboratory for extraction and MALDI ‐ TOF MS analysis. We also performed a literature review to compare various extraction methods.

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