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Bacterial viability differentially influences the immunomodulatory capabilities of potential host‐derived probiotics in the intestinal epithelial cells of Atlantic cod Gadus morhua
Author(s) -
Lazado C.C.,
Caipang C.M.A.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12414
Subject(s) - gadus , biology , immune system , microbiology and biotechnology , atlantic cod , immunology , fishery , fish <actinopterygii>
Aim This study explored the effect of heat inactivation on the immunomodulatory capabilities of two potential host‐derived probiotics ( GP 21 and GP 12) on the intestinal epithelial cells ( IEPC ) derived from Atlantic cod. Methods and Results The cells were isolated from the four segments of the gut, namely anterior intestine ( AI ), mid‐intestine ( MI ), posterior intestine ( PI ) and rectum ( RC ). The IEPC cultures were exposed to live or heat‐inactivated form of GP 21 and GP 12 for 24 h. The expression profiles of bacterial defence genes and cytokine genes in the probiotics‐exposed IEPC s showed differential patterns. Heat inactivation did not drastically affect the immunomodulatory properties of the probiotics, and this was explicitly typified by the stimulated expression of g‐type lysozyme, hepcidin, transferrin and metallothionein in both forms of the bacteria. There was no distinct expression pattern of the interleukin genes during bacterial exposure. This was in contrast to the chemokines where the expression of these genes in IEPC s was down‐regulated upon exposure to the heat‐inactivated probiotics. Although heat inactivation did not drastically affect the immunomodulatory capabilities of the probiotics, the live form elicited higher immune responses in the IEPC s in most cases. Conclusion This study showed that bacterial viability was a contributing influence, but not a major limiting factor on the immune‐related functions of the host‐derived probiotics in vitro . Significance and Impact of the Study GP 21 and GP 12 are beneficial host‐derived bacteria and could be utilized as candidate probiotics in cod aquaculture.