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Immunomodulating activity of exopolysaccharide‐producing L euconostoc mesenteroides strain NTM 048 from green peas
Author(s) -
Matsuzaki C.,
Kamishima K.,
Matsumoto K.,
Koga H.,
Katayama T.,
Yamamoto K.,
Hisa K.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12411
Subject(s) - library science , political science , computer science
Aims The present work was aimed to find novel probiotics to enhance the mucosal barrier function of humans. The effectiveness was evaluated in vitro and in vivo . Methods and Results Stimulation of I g A production in mucosal surfaces is one of the most beneficial traits of lactic acid bacteria ( LAB ) for enhancing the barrier. Therefore, 173 LAB strains were evaluated for the ability to induce I g A production using murine P eyer's patch cells. S train NTM 048 isolated from green peas showed the highest activity and was identified as L euconostoc mesenteroides subsp. mesenteroides . This strain was found to tolerate gastrointestinal digestion and produce large amounts of exopolysaccharides, which possess I g A ‐inducing activity. Dietary supplementation with NTM 048 induced a significant increase in the faecal I g A content and plasma I g A levels of BALB /c A mice. A gene expression analysis of P eyer's patch cells revealed that the transforming growth factor‐ β and activation‐induced cytidine deaminase genes were upregulated by NTM 048 intake. Conclusions Strain NTM 048 stimulates P eyer's patch cells to induce intestinal and systemic immune response, revealing the potential of NTM 048 as a probiotic for enhancing the mucosal barrier function. Significance and Impact of the Study This report demonstrates a food‐applicable L euconostoc mesenteroides strain secreting exopolysaccharide that shows high I g A ‐inducing ability.