Premium
A rapid colorimetric screening method for vanillic acid and vanillin‐producing bacterial strains
Author(s) -
Zamzuri N.A.,
AbdAziz S.,
Rahim R.A.,
Phang L.Y.,
Alitheen N.B.,
Maeda T.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12410
Subject(s) - vanillic acid , vanillin , ferulic acid , lignin , chemistry , strain (injury) , food science , biochemistry , chromatography , organic chemistry , biology , anatomy
Aim To isolate a bacterial strain capable of biotransforming ferulic acid, a major component of lignin, into vanillin and vanillic acid by a rapid colorimetric screening method. Methods and Results For the production of vanillin, a natural aroma compound, we attempted to isolate a potential strain using a simple screening method based on p H change resulting from the degradation of ferulic acid. The strain P seudomonas sp. AZ 10 UPM exhibited a significant result because of colour changes observed on the assay plate on day 1 with a high intensity of yellow colour. The biotransformation of ferulic acid into vanillic acid by the AZ 10 strain provided the yield ( Y p / s ) and productivity ( P r ) of 1·08 mg mg −1 and 53·1 mg L −1 h −1 , respectively. In fact, new investigations regarding lignin degradation revealed that the strain was not able to produce vanillin and vanillic acid directly from lignin; however, partially digested lignin by mixed enzymatic treatment allowed the strain to produce 30·7 mg l −1 and 1·94 mg l −1 of vanillic acid and biovanillin, respectively. Conclusions (i) The rapid colorimetric screening method allowed the isolation of a biovanillin producer using ferulic acid as the sole carbon source. (ii) Enzymatic treatment partially digested lignin, which could then be utilized by the strain to produce biovanillin and vanillic acid. Significance and Impact of the Study To the best of our knowledge, this is the first study reporting the use of a rapid colorimetric screening method for bacterial strains producing vanillin and vanillic acid from ferulic acid.