Premium
Identification of immunogenic proteins of C andida parapsilosis by serological proteome analysis
Author(s) -
Lee P.Y.,
Gam L.H.,
Yong V.C.,
Rosli R.,
Ng K.P.,
Chong P.P.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12408
Subject(s) - candida parapsilosis , biology , proteome , antigen , microbiology and biotechnology , pathogen , antigenicity , serology , candida albicans , proteomics , virology , antibody , immunology , gene , biochemistry
Aims Systemic candidiasis is the leading fungal bloodstream infection, and its incidence has been on the rise. Recently, C andida parapsilosis has emerged as an increasingly prevalent fungal pathogen, but little is known about its antigenic profile. Hence, the current work was performed to discover immunogenic proteins of C . parapsilosis using serological proteome analysis. Methods and Results Cell wall proteins extracted from C . parapsilosis were resolved by two‐dimensional electrophoresis followed by immunoblotting using antisera from experimentally infected mice. Mass spectrometry analysis of the 32 immunoreactive protein spots resulted in the identification of 12 distinct proteins. Among them, 11 proteins were known antigens of Candida albicans, whereas Idh2p was identified for the first time as an immunogenic protein of Candida species. Recombinant Idh2p was expressed in Escherichia coli, and its antigenicity was verified by immunoblot analysis. Conclusions An immunoproteomic approach was successfully applied to identify immunogenic proteins of C . parapsilosis , with Idh2p as a novel candidate antigen. The identified antigens may serve as potential biomarkers for development of diagnostic assay and/or vaccine for C. parapsilosis . Significance and Impact of the Study This work represents the first immunoproteomic analysis of C. parapsilosis, which provides new insights into host–pathogen interactions and pathogenesis of C. parapsilosis . The immunogenic proteins could be studied as biomarker candidates for C. parapsilosis .