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Exploring the potential of B urkholderia sacchari to produce polyhydroxyalkanoates
Author(s) -
Mendonça T.T.,
Gomez J.G.C.,
Buffoni E.,
Sánchez Rodriguez R.J.,
Schripsema J.,
Lopes M.S.G.,
Silva L.F.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12406
Subject(s) - humanities , library science , philosophy , computer science
Aim Evaluation of the capability of B urkholderia sacchari to incorporate different monomers into polyhydroxyalkanoates ( PHA ). Methods and Results Thirty different carbon sources were evaluated as cosubstrates for B . sacchari growing on glucose with the intention to promote the incorporation of different monomers into the PHA produced by this species. With odd‐numbered fatty acids, incorporation of the 3 HV monomer was achieved, up to 65 mol% in the case of valerate. With 4‐hydroxybutyrate, incorporation of 4 HB was obtained, representing 9·1 mol%. With hexanoic acid, the production of P 3 HB ‐ co ‐3 HH x was achieved, containing up to 1·6 mol% of 3 HH x. The molar fraction of 3 HH x was found to be dependent on the ratio of glucose to hexanoic acid supplied. Metabolic flux analysis revealed a high efficiency of B . sacchari in converting carbon sources into P 3 HB ‐ co ‐3 HH x. Nevertheless, hexanoic acid was only poorly converted to 3 HH x. Conclusions B urkholderia sacchari is able to incorporate 3 HV , 4 HB and 3 HH x in PHA containing mainly 3 HB . The 3 HH x content of P 3 HB ‐ co ‐3 HH x can be controlled by varying the glucose to hexanoic acid ratio. B urkholderia sacchari is highly efficient in converting carbon sources into PHA ; however, only 2% of the hexanoic acid supplied could be converted to 3 HH x. Significance and Impact of the Study This is the first report describing an approach to modulate the composition of P3 HB ‐ co ‐3 HH x produced by bacteria using mixtures of carbohydrate and hexanoic acid as carbon source.