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Degradation of dibenzofuran via multiple dioxygenation by a newly isolated A grobacterium sp. PH ‐08
Author(s) -
Le T.T.,
Murugesan K.,
Nam I.H.,
Jeon J.R.,
Chang Y.S.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12403
Subject(s) - dibenzofuran , chemistry , dibenzothiophene , biodegradation , agrobacterium , biotransformation , strain (injury) , dioxygenase , rhizobiaceae , catechol , stereochemistry , cometabolism , oxygenase , degradation (telecommunications) , biochemistry , enzyme , bacteria , gene , transformation (genetics) , organic chemistry , biology , bioremediation , genetics , symbiosis , anatomy , catalysis , telecommunications , computer science
Aims To demonstrate the biodegradation of dibenzofuran ( DF ) and its structural analogs by a newly isolated A grobacterium sp. PH ‐08. Methods and Results To assess the biodegradation potential of newly isolated A grobacterium sp. PH ‐08, various substrates were evaluated as sole carbon sources in growth and biotransformation experiments. ESI LC ‐ MS / MS analysis revealed the presence of angular degrading by‐products as well as lateral dioxygenation metabolites in the upper pathway. The metabolites in the lower pathway also were detected. In addition, the cometabolically degraded daughter compounds of DF ‐related compounds such as BP and dibenzothiophene ( DBT ) in dual substrate degradation were observed. Strain PH ‐08 exhibited the evidence of meta‐cleavage pathway as confirmed by the activity and gene expression of catechol‐2,3‐dioxygenase. Conclusions Newly isolated bacterial strain, A grobacterium sp. PH ‐08, grew well with and degraded DF via both angular and lateral dioxygenation as demonstrated by metabolites identified through ESI LC ‐ MS / MS and GC ‐ MS analyses. The other heterocyclic pollutants were also cometabolically degraded. Significance and Impact of the Study Few reports have described the complete degradation of DF by a cometabolic lateral pathway. Our study demonstrates the novel results that the newly isolated strain utilized the DF as a sole carbon source and mineralized it via multiple dioxygenation.