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Preventive effects of an original combination of grape seed polyphenols with amine fluoride on dental biofilm formation and oxidative damage by oral bacteria
Author(s) -
Furiga A.,
Roques C.,
Badet C.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12395
Subject(s) - polyphenol , chemistry , biofilm , antioxidant , food science , streptococcus mutans , trolox , phenols , grape seed extract , saliva , trolox equivalent antioxidant capacity , antibacterial activity , bacteria , microbiology and biotechnology , biochemistry , biology , antioxidant capacity , medicine , alternative medicine , pathology , genetics
Aims To investigate the preventive effects of an original combination of a grape seed extract ( GSE ) with an amine fluoride ( F luorinol ® ) on dental plaque formation and oxidative damage caused by oral bacteria. Methods and Results The antibacterial activity of the compounds was assessed using the broth macrodilution method, and their antiplaque activity was evaluated on a multispecies biofilm grown on saliva‐coated hydroxyapatite discs. The effect on glucosyltransferases activity was analysed through reductions in the overall reaction and the quantity of insoluble glucan synthesized. The combination of 2000 μ g ml −1 of GSE with 10·2 mg ml −1 of Fluorinol ® significantly decreased the biofilm formation (up to 4·76 log 10 of reduction) and inhibited by 97·4% the insoluble glucan synthesis by glucosyltransferases. The antioxidant activity of this combination, alone or incorporated into a formulated mouthwash (Eludril daily ® ), was determined using the Trolox equivalent antioxidant capacity assay ( TEAC ), and both showed significantly greater antioxidant capacity than vitamin C . Conclusions The GSE / F luorinol ® combination showed both a significant antiplaque activity and an important antioxidant capacity in vitro , without any bactericidal effects. Significance and Impact of the Study This is, to our knowledge, the first report on the properties of an original combination of a polyphenolic extract with amine fluoride that could be used for the prevention of oral diseases and oxidative damage associated.