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Effects of light on secondary metabolism and fungal development of F usarium graminearum
Author(s) -
Kim H.,
Son H.,
Lee Y.W.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12381
Subject(s) - fusarium , secondary metabolism , fungi imperfecti , biology , mycotoxin , metabolism , fungal growth , botany , microbiology and biotechnology , biochemistry , biosynthesis , enzyme
Aims The objectives of this study were (i) to characterize white‐collar ( WC ) orthologues of the filamentous fungus F usarium graminearum , (ii) to investigate light‐responsive phenotypes by the deletion of F gwc‐1 and F gwc‐2 genes and (iii) to examine the roles of those genes in constant light and darkness in relation to secondary metabolite synthesis and development. Methods and Results Production of secondary metabolites and asexual/sexual development of deletion mutants, Δ F gwc‐1 and Δ F gwc‐2 , were assessed in constant light and darkness compared to the wild‐type strain. The results showed that deletion of F gwc‐1 and F gwc‐ 2 impaired early onset of carotenogenesis, photoreactivation and the maturity of perithecia during sexual development. Conidiation of the Δ F gwc‐1 and Δ F gwc‐2 mutants was derepressed in constant light, but not in darkness. Moreover, the individual mutants produced more aurofusarin and trichothecenes than the wild‐type strain in both constant light and darkness. Conclusions Both F gwc‐1 and F gwc‐2 are required for light‐dependent processes in F . graminearum, whereas light‐independent processes such as aurofusarin and trichothecene biosynthesis are derepressed by deletion of these genes. Thus, F gwc‐1 and F gwc‐2 play roles as positive and negative regulators, depending on the requirement of light for biological activity. Significance and Impact of the Study These results will extend the knowledge of the photobiology of F usarium graminearum and will increase current understanding of light regulatory mechanisms mediated by white collar in secondary metabolism and fungal development.