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Transcriptional regulation of enniatins production by Fusarium avenaceum
Author(s) -
Fanelli F.,
Ferracane R.,
Ritieni A.,
Logrieco A.F.,
Mulè G.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12371
Subject(s) - fusarium , biology , transcriptional regulation , transcription (linguistics) , gene , gene expression , regulation of gene expression , biosynthesis , microbiology and biotechnology , secondary metabolism , biochemistry , genetics , linguistics , philosophy
Aims The aim of this study was to analyse the transcriptional regulation of enniatins ( EN s) production in F usarium avenaceum . Methods and Results We develop a new method to quantify EN s in FDM agar medium. We performed an LC / MS / MS analysis to evaluate enniatin A, A1, B, B1 and B4 production by seven F . avenaceum strains and, in a time‐course experiment, by ITEM 3404 to analyse the transcriptional regulation of the esyn1 gene. The expression profile, achieved by R eal time reverse transcriptase assay, showed an activation of gene transcription at the seventh day of incubation, corresponding to the higher increase of total EN s production. Enniatin B was the most abundant EN s analogues, representing the 90% of total EN s. The relative percentage of EN s remained unaltered during the experiment. Conclusions We reported a transcriptional regulation of esyn1 responsible for the modulation of EN s biosynthesis. Significance and Impact of the Study Enniatins are cyclic depsipeptides metabolites with a wide range of biological activities. They are also widespread contaminants in grains and cereals due to infection by enniatin‐producing F usarium species. This is the first article describing the transcriptional regulation of esyn1 gene that modulates EN s production in F usarium avenaceum and provides new knowledge about the molecular mechanism underlying the biosynthesis of these important fungal metabolites in this toxigenic fungal species.