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Molecular diversity and distribution of anammox community in sediments of the D ongjiang R iver, a drinking water source of H ong K ong
Author(s) -
Sun W.,
Xu M.Y.,
Wu W.M.,
Guo J.,
Xia C.Y.,
Sun G.P.,
Wang A.J.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12367
Subject(s) - anammox , candidatus , 16s ribosomal rna , bacteria , biology , community structure , ecology , environmental chemistry , chemistry , denitrification , nitrogen , denitrifying bacteria , organic chemistry , genetics
Aims The aim of this study was to characterize anaerobic ammonium oxidation (anammox) community in sediments of the D ongjiang R iver, a drinking water source of H ong K ong. Methods and Results The diversity and distribution of the anammox community were investigated based on a comparative analyses of 16 S r RNA and hydrazine oxidation ( hzo ) genes of anammox bacteria. C andidatus B rocadia and two new anammox bacterial clusters were detected based on phylogenetic analysis of 16 S r RNA genes. In contrast, the targeting of hzo genes indicated the presence of only C andidatus Jettenia with four different clusters. It was found that the sequence diversities of hzo genes were higher than those of the 16 S r RNA genes. The abundance of anammox bacteria varied significantly among the sediment samples based on q PCR . Pearson correlation analysis indicated that nitrite concentration was the key factor influencing the abundance of anammox bacteria. The redundance analysis ( RDA ) confirmed that the combination of the contents of nitrite and nitrate, and the ratio of total nitrogen vs total carbon ( TN / TC ) had significant impact on the anammox bacterial community structure. Conclusions The results revealed that the diverse anammox bacteria were present in sediments of the D ongjiang R iver, and the community structures were associated with varied environmental factors caused by urban pollutant invasion. Significance and Impact of the Study This is the first report about the distribution of anammox bacterial community in sediments of the D ongjiang R iver, which provides helpful information of anammox niche specificity and influencing factors in the river ecosystem.