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Investigation of potential markers of acid resistance in L actobacillus plantarum by comparative proteomics
Author(s) -
Hamon E.,
Horvatovich P.,
Marchioni E.,
AoudéWerner D.,
Ennahar S.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12339
Subject(s) - lactobacillus plantarum , proteomics , microbiology and biotechnology , lactobacillus , biology , bacteria , lactobacillaceae , computational biology , biochemistry , lactic acid , genetics , gene
Aims To investigate cell determinants of resistance to gastric acidity in L actobacillus plantarum using comparative proteomics. Methods and Results Among ten L act. plantarum strains that were tested for their acid resistance in vitro , three strains with different phenotypes were selected for comparative proteomic analysis: LC 804 (resistant), CIP A159 (intermediate) and CECT 4185 (sensitive). Constitutive differences between whole‐cell proteomes of the three strains were studied. Among the differentially expressed proteins between strains, 17 have previously been reported to be involved in acid resistance processes. The effect of a low‐p H exposure on these proteomic patterns was investigated, which led to the identification of five putative determinants of acid resistance (heat‐shock protein G rp E , methionine synthase and 30 S ribosomal protein S 2) or sensitivity (phosphotransacetylase and adenylosuccinate synthase). Analysis also revealed three additional proteins involved in cell envelope biogenesis (3‐oxoacyl‐synthase II, dTDP ‐glucose 4,6‐dehydratase and d TDP ‐4‐dehydrorhamnose 3,5‐epimerase) likely to be key factors of intrinsic acid tolerance in L act. plantarum . Conclusions The approach used in this study enabled the identification of potential markers of acid tolerance in L act. plantarum, which may serve for phenotyping and screening purposes. Significance and Impact of the Study The present work represents a further step towards the identification of bacterial biomarkers for each particular probiotic feature.