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Evaluation of anti‐infective potential of a tribal folklore O dina wodier R oxb against some selected microbes and herpes simplex virus associated with skin infection
Author(s) -
Ojha D.,
Mukherjee H.,
Ghosh S.,
Bag P.,
Mondal S.,
Chandra N.S.,
Mondal K.C.,
Samanta A.,
Chakrabarti S.,
Chattopadhyay D.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12330
Subject(s) - antimicrobial , microbiology and biotechnology , biology , staphylococcus aureus , bacteria , candida albicans , klebsiella pneumonia , genetics
Aim To evaluate the in vitro antimicrobial activity of aqueous and methanol extracts of O dina wodier bark ( OWB ) , a folk medicine, against representative bacteria, fungi and herpes simplex virus ( HSV ) associated with skin infections. Methods and Results The OWB extract(s) was found to inhibit the isolates of S taphylococcus aureus , B acillus subtilis, P seudomonas aeruginosa , K lebsiella pneumonia , E scherichia coli at an MIC of 256–5000 μg ml −1 and C andida albicans at and above 4000 μg ml −1 by agar and broth dilution assays. The growth curve of S taph. aureus revealed the highest activity within 2–6 h of methanol extract ( ME ) exposure. Interestingly, the MTT and plaque reduction assay showed that the extracts can inhibit HSV ‐1 and HSV ‐2 at EC 50 of 22·4 and 28·8 μg ml −1 , with Selectivity index of 11·7–15. While the time kinetic and binding assays demonstrated that the ME at 50 μg ml −1 prevents viral attachment into V ero cells. Phytochemical and HPLC analysis of ME revealed the presence of flavonoids, phytosterols, saponins and tannins including the pseudotannin chlorogenic acid. Conclusion The traditional use of OWB for the management of skin infections has scientific basis. Significance and Impact of the Study This study demonstrated the antimicrobial potential of OWB on selected isolates of bacteria, fungi and HSV , associated with skin infections.