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Immune responses of chickens inoculated with recombinant L actobacillus expressing the haemagglutinin of the avian influenza virus
Author(s) -
Wang Z.,
Yu Q.,
Fu J.,
Liang J.,
Yang Q.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12325
Subject(s) - christian ministry , china , agriculture , library science , biology , microbiology and biotechnology , political science , ecology , law , computer science
Abstract Aims To develop a safe, effective and convenient vaccine for the prevention of highly pathogenic avian influenza (HPAI), we have successfully constructed a recombinant lactobacillus (LDL17‐ pH ) that expresses the foreign HPAI protein, haemagglutinin 1 (HA 1 ). Methods and Results The mucosal and systemic immune responses that are triggered by LDL17‐ pH following the oral administration to 10‐day‐old chickens were evaluated. The results showed that LDL17‐ pH could significantly increase the specific anti‐HA 1 IgA antibody level in the mucosa and the anti‐HA 1 IgG level in sera. Tissues were isolated from trachea and Peyer's patches(PPs)and caecal tonsils of chickens, and gene expression was analysed via real‐time quantitative PCR. Conclusions The results showed that LDL17‐ pH could significantly induce the specific anti‐HA 1 IgA antibody level in the trachea and intestine and the specific anti‐HA 1 IgG antibody level in the serum ( P < 0·05). Additionally, LDL17‐ pH was in the capacity to induce the expression of cytokines IFN‐γ, TLR‐2 and AvBD‐9 in the PPs and caecal tonsils. Most importantly, the chickens that were immunized with LDL17‐ pH were protected against lethal challenge of the H5N1 virus to some extent. Significance and Impact of the Study Therefore, LDL17‐ pH could be a promising oral vaccine candidate against HPAI.