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Kannurin, a novel lipopeptide from Bacillus cereus strain AK 1: isolation, structural evaluation and antifungal activities
Author(s) -
Ajesh K.,
Sudarslal S.,
Arunan C.,
Sreejith K.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12324
Subject(s) - lipopeptide , bacillus cereus , microbiology and biotechnology , strain (injury) , isolation (microbiology) , biology , antifungal , bacteria , genetics , anatomy
Abstract Aim This study was performed to isolate and characterize novel antifungal lipopeptide from B acillus cereus . Methods and Results Elucidation of its chemical structure was carried out by electrospray ionization mass spectra ( ESI ‐ MS ) and F ourier transform infrared spectroscopy ( FT ‐ IR ). The compound is a cyclic heptapeptide and composed of amino acids, Leu–Asp–Val–Leu–Leu–Leu–Leu. The in vitro activity of K annurin against various pathogenic yeasts was assessed by CLSI M27‐A and moulds by M38‐A. It demonstrated broad‐spectrum, fungicidal activity against clinically relevant yeasts and moulds. Kannurin exhibited low haemolytic activity and remained active over a wide pH and temperature range. In addition, K annurin did not bind with melanin particles and was as active in inhibiting biofilms. Conclusions An antifungal surfactin‐like lipopeptide produced by Bacillus cereus strain AK 1 was purified and chemically characterized. We propose to name this lipopeptide compound ‘Kannurin’. To our knowledge, this is the first report of Bacillus cereus producing surfactin‐like lipopeptide antibiotic with stronger antifungal activity. Significance and Impact of the Study Our results provide a valuable contribution towards a better understanding of the lipopeptide of B acillus cereus . Moreover, it raises the possibility of using as an alternative antibiotic in clinical medicine.