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Inhibition of initial adhesion of oral bacteria through a lectin from B auhinia variegata L . var. variegata expressed in E scherichia coli
Author(s) -
Klafke G.B.,
Borsuk S.,
Gonçales R.A.,
Arruda F.V.S.,
Carneiro V.A.,
Teixeira E.H.,
Coelho da Silva A.L.,
Cavada B.S.,
Dellagostin O.A.,
Pinto L.S.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12318
Subject(s) - streptococcus sobrinus , lectin , streptococcus mutans , recombinant dna , biology , microbiology and biotechnology , affinity chromatography , agglutination (biology) , escherichia coli , adhesion , bacteria , biofilm , biochemistry , chemistry , enzyme , antibody , gene , genetics , organic chemistry , immunology
Aims The aim of the present work was to study the in vitro effect of native and recombinant B auhinia variegata var. variegata lectins in inhibiting early adhesion of S treptococcus mutans , S treptococcus sanguis and S treptococcus sobrinus to experimentally acquired pellicle. Methods and Results Native lectin from B . variegata ( BVL ) was purified by affinity chromatography of extract of seeds. The recombinant lectin ( rBVL ‐ I ) was expressed in E . coli strain BL 21 ( DE 3) from a genomic clone encoding the mature B . variegata lectin gene using the vector pAE ‐ bvl I . Recombinant protein deposited in inclusion bodies was solubilized and subsequently purified by affinity chromatography. The rBVL ‐I was compared to BVL for agglutination of erythrocytes and initial adherence of oral bacteria on a saliva‐coated surface. The results revealed that rBVL ‐I acts similarly to BVL for agglutination of erythrocytes. Both lectins showed adhesion inhibition effect on S tep. sanguis , S tep. mutans and S tep. sobrinus . Conclusion We report, for the first time, the inhibition of early adhesion of oral bacteria by a recombinant lectin. Significance and Impact of the Study Our results support the proposed biotechnological application of lectins in a strategy to reduce development of dental caries by inhibiting the initial adhesion and biofilm formation.