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Fatty acid oxidation products (‘green odour’) released from perennial ryegrass following biotic and abiotic stress, potentially have antimicrobial properties against the rumen microbiota resulting in decreased biohydrogenation
Author(s) -
Huws S.A.,
Scott M.B.,
Tweed J.K.S.,
Lee M.R.F.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12314
Subject(s) - rumen , biology , terminal restriction fragment length polymorphism , food science , bacteria , antimicrobial , red clover , microbiology and biotechnology , botany , fermentation , biochemistry , restriction fragment length polymorphism , polymerase chain reaction , genetics , gene
Aims In this experiment, we investigated the effect of ‘ g reen o dour’ products typical of those released from fresh forage postabiotic and biotic stresses on the rumen microbiota and lipid metabolism. Methods and Results Hydroperoxyoctadecatrienoic acid ( HP ), a combination of salicylic and jasmonic acid ( T ), and a combination of both ( HPT ) were incubated in vitro in the presence of freeze‐dried ground silage and rumen fluid, under rumen‐like conditions. 16S rRNA (16S cDNA ) HaeIII ‐based terminal restriction fragment length polymorphism‐based ( T‐RFLP ) dendrograms, canonical analysis of principal coordinates graphs, peak number and S hanon‐ W einer diversity indices show that HP , T and HPT likely had antimicrobial effects on the microbiota compared to control incubations. Following 6 h of in vitro incubation, 15·3% of 18:3 n‐3 and 4·4% of 18:2 n‐6 was biohydrogenated in control incubations, compared with 1·3, 9·4 and 8·3% of 18:3 n‐3 for HP , T and HPT treatments, respectively, with negligible 18:2 n‐6 biohydrogenation seen. T ‐ RFLP peaks lost due to application of HP , T and HPT likely belonged to as yet uncultured bacteria within numerous genera. Conclusions H ydroperoxyoctadecatrienoic acid, T and HPT released due to plant stress potentially have an antimicrobial effect on the rumen microbiota, which may explain the decreased biohydrogenation in vitro . Significance and Impact of the Study These data suggest that these volatile chemicals may be responsible for the higher summer n‐3 content of bovine milk.