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A diversity profile from the staphylococcal community on atopic dermatitis skin: a molecular approach
Author(s) -
Soares J.,
Lopes C.,
Tavaria F.,
Delgado L.,
Pintado M.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12296
Subject(s) - microbiology and biotechnology , staphylococcus aureus , biology , atopic dermatitis , staphylococcus epidermidis , coagulase , staphylococcal infections , multiplex polymerase chain reaction , virulence , toxic shock syndrome , staphylococcus , superantigen , staphylococcus haemolyticus , enterotoxin , polymerase chain reaction , bacteria , immunology , gene , genetics , escherichia coli
Aims The aim of this study was to determine the biodiversity of the skin staphylococcal community from patients with atopic dermatitis ( AD ) and superantigen ( SA g) detection from S taphylococcus aureus isolates. Methods and Results In this study, we developed a novel multiplex PCR that allows the identification and discrimination of bacteria belonging to the S taphylococcus genus both S taph. aureus and coagulase‐negative S taphylococcus − S taph. capitis , S taph. epidermidis , S taph. haemolyticus and S taph. hominis isolated from the skin of patients with AD . In addition, a multiplex PCR assay that allows the rapid screening of the 19 genes that encode staphylococcal enterotoxins ( SE s), SE ‐like toxins and toxic shock syndrome toxin‐1 was also performed and applied in S taph. aureus isolates. The microflora of the skin of patients with AD was dominated by S taph. aureus (69 isolates, 35·6%) followed by S taph. epidermidis (59 isolates, 30·4%) species. The SElM and SElN genes were the most frequently detected in our study (15 isolates, 71·4%), followed by SEG and SElO (14 isolates, 66·7%). Conclusions Our molecular‐based approach successfully identified the staphylococcal microflora that was relatively specific to patients with AD . Considering skin colonization and expression of virulence factors, the S taph. aureus may play a relevant role in AD pathophysiology. Significance and Impact of the Study This ability to classify disease‐related microbial species provides new insights into the relevance of those microbes in human disorders.