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In vitro degradation of oxalate by recombinant L actobacillus plantarum expressing heterologous oxalate decarboxylase
Author(s) -
Anbazhagan K.,
Sasikumar P.,
Gomathi S.,
Priya H.P.,
Selvam G.S.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12269
Subject(s) - lactobacillus plantarum , recombinant dna , in vitro , oxalate , heterologous , microbiology and biotechnology , degradation (telecommunications) , chemistry , biochemistry , biology , bacteria , lactic acid , gene , computer science , organic chemistry , genetics , telecommunications
Aim The aim of the present study is to constitutively express heterologous oxalate decarboxylase (OxdC) in Lactobacillus plantarum and to examine its ability to degrade oxalate in vitro for their future therapy against enteric hyperoxaluria. Method and Results In this study, we generated a recombinant strain of Lb. plantarum to constitutively overexpress B. subtilis oxalate decarboxylase ( oxdC ) using a host lactate dehydrogenase promoter (P ldhL ). The recombinant Lb. plantarum was able to degrade more than 90% oxalate compared to 15% by the wild type. In addition, the recombinant strain also had higher tolerance up to 500 mmol l −1 oxalate. Conclusion We developed a recombinant Lb. plantarum NC 8 that constitutively expressed heterologous oxalate decarboxylase and degraded oxalate efficiently under in vitro conditions. Significance and impact of study The long‐term aim is to develop an efficient strain for future therapy against oxalosis.