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Enterocin TW 21, a novel bacteriocin from dochi ‐isolated E nterococcus faecium D 081821
Author(s) -
Chang S.Y.,
Chen Y.S.,
Pan S.F.,
Lee Y.S.,
Chang C.H.,
Chang C.H.,
Yu B.,
Wu H.C.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12265
Subject(s) - bacteriocin , enterococcus faecium , microbiology and biotechnology , biology , listeria monocytogenes , ammonium sulfate precipitation , listeria , peptide sequence , biochemistry , bacteria , antimicrobial , size exclusion chromatography , gene , genetics , antibiotics , enzyme
Aims Purification and characterization of a novel bacteriocin produced by strain E nterococcus faecium D 081821. Methods and Results E nterococcus faecium D 081821, isolated from the traditional Taiwanese fermented food dochi (fermented black beans), was previously found to produce a bacteriocin against L isteria monocytogenes and some G ram‐positive bacteria. This bacteriocin, termed enterocin TW 21, was purified from culture supernatant by ammonium sulfate precipitation, S ep‐ P ak C 18 cartridge, ion‐exchange and gel filtration chromatography. Mass spectrometry analysis showed the mass of the peptide to be approximately 5300·6 Da. The N ‐terminal amino acid sequencing yielded a partial sequence NH 2 ‐ ATYYGNGVY x NTQK by Edman degradation, and it contains the consensus class II a bacteriocin motif YGNGV in the N ‐terminal region. The open reading frame ( ORF ) encoding the bacteriocin was identified from the draft genome sequence of E nterococcus faecium D 081821, and sequence analysis of this peptide indicated that enterocin TW 21 is a novel bacteriocin. Conclusions Enterococcus faecium D 081821 produced a bacteriocin named enterocin TW 21, the molecular weight and amino acid sequence both revealed it to be a novel bacteriocin. Significant and Impact of Study A new member of class II a bacteriocin was identified. This bacteriocin shows great inhibitory ability against L . monocytogenes and could be applied as a natural food preservative.