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Presence of acyl‐homoserine lactones in 57 members of the Vibrionaceae family
Author(s) -
Purohit A.A.,
Johansen J. A.,
Hansen H.,
Leiros H.K.S.,
Kashulin A.,
Karlsen C.,
Smalås A.,
Haugen P.,
Willassen N.P.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12264
Subject(s) - vibrionaceae , biology , photobacterium , quorum sensing , vibrio , microbiology and biotechnology , bacteria , homoserine , enterobacteriaceae , biochemistry , genetics , escherichia coli , gene , biofilm
Aims The aim of this study was to use a sensitive method to screen and quantify 57 Vibrionaceae strains for the production of acyl‐homoserine lactones ( AHL s) and map the resulting AHL profiles onto a host phylogeny. Methods and Results We used a high‐performance liquid chromatography–tandem mass spectrometry ( HPLC ‐ MS / MS ) protocol to measure AHL s in spent media after bacterial growth. First, the presence/absence of AHL s (qualitative analysis) was measured to choose internal standard for subsequent quantitative AHL measurements. We screened 57 strains from three genera ( Aliivibrio , Photobacterium and Vibrio ) of the same family (i.e. Vibrionaceae ). Our results show that about half of the isolates produced multiple AHL s, typically at 25–5000 nmol l −1 . Conclusions This work shows that production of AHL quorum sensing signals is found widespread among Vibrionaceae bacteria and that closely related strains typically produce similar AHL profiles. Significance and Impact of the Study The AHL detection protocol presented in this study can be applied to a broad range of bacterial samples and may contribute to a wider mapping of AHL production in bacteria, for example, in clinically relevant strains.