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Purification, characterization and preparation immunomatrixes of S ‐layer proteins of T hermobifida fusca
Author(s) -
Pervaiz S.,
Shaheen T.,
Shaheen S.,
Dar N.,
Samra Z.Q.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12259
Subject(s) - s layer , toxin , guanidine , chemistry , bradford protein assay , chromatography , biochemistry , molecular mass , immunogold labelling , antibody , microbiology and biotechnology , biology , enzyme , gene , immunology
Aim S‐layer proteins are considered as a good nanocarrier due to their binding and self‐assembled properties. These can be used to prepare the immunomatrixes for the removal of toxins from the samples. Methods and Results Two S ‐layer proteins 70 and 40 kDa of thermophilic Thermobifida fusca were extracted with guanidine hydrochloride and purified. Antibodies against S‐layer proteins were developed, and their monospecificity was checked. Immunogold labelling indicated that these are surface proteins. Immunomatrixes (70‐ SLIM , 40 SLIM ) were prepared by covalently immobilizing S‐layer proteins in microwell and further conjugated with anti‐ Staphylococcus aureus enterotoxin B ( SEB ) antibodies. The binding of 70 and 40 kDa proteins was observed nearly 7·0 μg cm −1 to binding area, and the conjugation with anti‐ SEB antibodies was found 1·22 μg μg −1 of 70 kDa and 0·875 μg μg −1 of 40 kDa. The average binding and elution of pure SEB toxin on 70‐ SLIM and 40‐ SLIM was 5·0 μg SEB toxin. The SEB toxin in milk samples was also removed on immunomatrixes successfully. Conclusion It is the first report, and this study shows that the thermophilic S ‐layer proteins can be used to prepare the immunomatrixes. Significance and Impact of Study Information in this study can be used to design the strategies for the removal of biologically important materials or toxins from samples.