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Bioconversion of elephant grass ( P ennisetum purpureum ) acid hydrolysate to bacterial cellulose by G luconacetobacter xylinus
Author(s) -
Yang X.Y.,
Huang C.,
Guo H.J.,
Xiong L.,
Li Y.Y.,
Zhang H.R.,
Chen X.D.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12255
Subject(s) - bioconversion , pennisetum purpureum , hydrolysate , cellulose , food science , bacterial cellulose , crystallinity , fermentation , chemistry , hydrolysis , sugar , biology , botany , biochemistry , dry matter , crystallography
Aims To evaluate the possibility of elephant grass acid hydrolysate converting into bacterial cellulose ( BC ) produced by G luconacetobacter xylinus CH 001 and to characterize the morphology and structure of the cellulose produced. Methods and Results Acid‐hydrolysed and detoxified elephant grass acid hydrolysate was inoculated with G . xylinus CH 001. After 14 days of static fermentation, about 6·4 g l −1 of BC could be generated. Meanwhile, 60·4% (w/w) of BC yield on sugar consumption was obtained. Scanning electron micrographs illustrated that the network of cellulose fibres became denser, and the diameter changed with the growth. FT ‐ IR spectra showed almost same results for all the BC samples collected on different culture time. X‐ray diffractograms demonstrated that the crystalline form of BC was cellulose I, the crystallinity increased to 53·58%, and the crystallinity index reached up to 99%. Conclusions Elephant grass acid hydrolysate could be utilized efficiently for BC production by G . xylinus CH 001. Structure analysis on the cellulose produced showed its potential of being excellent material for further application. Significance and Impact of the Study Our studies for the first time examined the bioconversion of low‐cost elephant grass into high‐value BC and the changes in its morphology and structure following the culture time.