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Decontamination of materials contaminated with B acillus anthracis and B acillus thuringiensis A l H akam spores using PES ‐ S olid, a solid source of peracetic acid
Author(s) -
Buhr T.L.,
Wells C.M.,
Young A.A.,
Minter Z.A.,
Johnson C.A.,
Payne A.N.,
McPherson D.C.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12253
Subject(s) - spore , peracetic acid , bacillus anthracis , bacillus thuringiensis , microbiology and biotechnology , bacillus (shape) , human decontamination , bacillales , chemistry , food science , biology , bacteria , hydrogen peroxide , biochemistry , waste management , genetics , bacillus subtilis , engineering
Aims To develop test methods and evaluate survival of B acillus anthracis A mes, B . anthracis ∆Sterne and B . thuringiensis A l H akam spores after exposure to PES ‐ S olid (a solid source of peracetic acid), including PES ‐ S olid formulations with bacteriostatic surfactants. Methods and Results Spores (≥7 logs) were dried on seven different test materials and treated with three different PES ‐Solid formulations (or preneutralized controls) at room temperature for 15 min. There was either no spore survival or less than 1 log (<10 spores) of spore survival in 56 of 63 test combinations (strain, formulation and substrate). Less than 2·7 logs (<180 spores) survived in the remaining seven test combinations. The highest spore survival rates were seen on water‐dispersible chemical agent resistant coating ( CARC ‐W) and Naval ship topcoat (NTC). Electron microscopy and Coulter analysis showed that all spore structures were intact after spore inactivation with PES ‐Solid. Conclusions Three PES ‐Solid formulations inactivated Bacillus spores that were dried on seven different materials. Significance and Impact of the Study A test method was developed to show that PES ‐Solid formulations effectively inactivate Bacillus spores on different materials.