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The expression of adhesin EF ‐Tu in response to mucin and its role in L actobacillus adhesion and competitive inhibition of enteropathogens to mucin
Author(s) -
Dhanani A.S.,
Bagchi T.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12249
Subject(s) - mucin , microbiology and biotechnology , bacterial adhesin , biology , escherichia coli , biochemistry , gene
Aims To analyse the expression of EF ‐Tu in L actobacillus strains with response to mucin exposure and its role in interfering with adhesion of enteropathogens to mucin. Methods and Results The Lactobacillus strains were analysed for their ability to adhere to immobilized mucin in microtiter plates. Lactobacillus delbrueckii M and L actobacillus plantarum CS 24·2 showed statistically significant adhesion to mucin, which was similar to L actobacillus rhamnosus GG , the best binding probiotic strain. L actobacillus rhamnosus GG , L act. delbrueckii M, L act. plantarum CS 23 and Lact. plantarum CS 24·2 were able to effectively antagonize the adhesion of Escherichia coli and S almonella enterica serovar Typhi to mucin. In the presence of L actobacillus adhesin – EF ‐Tu, the adhesion of L act. delbrueckii M and the strains of L act. plantarum to mucin was significantly inhibited. Similarly, EF ‐Tu also reduced the adhesion of enteropathogens to mucin. Furthermore, the relative fold change in gene expression analysis showed significant up‐regulation of EF ‐Tu gene in the strains of L act. plantarum and L act. delbrueckii M when exposed to mucin for 3 h. Conclusions The study shows the significant role of EF ‐Tu in lactobacilli adhesion and enteropathogens inhibition. Significance and Impact of the Study The study suggests EF ‐Tu as an important factor linked to the L actobacillus adhesion as well as enteropathogen inhibition. L actobacillus plantarum CS 23 and L act. plantarum CS 24·2 can be used as potential probiotic strains.