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Bacillus amyloliquefaciens ssp. plantarum strains as potential protective starter cultures for the production of Bikalga , an alkaline fermented food
Author(s) -
Compaoré C.S.,
Nielsen D.S.,
SawadogoLingani H.,
Berner T.S.,
Nielsen K.F.,
Adimpong D.B.,
Diawara B.,
Ouédraogo G.A.,
Jakobsen M.,
Thorsen L.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12214
Subject(s) - bacillus amyloliquefaciens , surfactin , antimicrobial , microbiology and biotechnology , hibiscus sabdariffa , bacillus cereus , biology , lactobacillus plantarum , food science , lipopeptide , bacillus (shape) , fermentation , bacteria , bacillus subtilis , lactic acid , genetics
Aims To identify and screen dominant Bacillus spp. strains isolated from Bikalga , fermented seeds of Hibiscus sabdariffa for their antimicrobial activities in brain heart infusion ( BHI ) medium and in a H. sabdariffa seed‐based medium. Further, to characterize the antimicrobial substances produced. Methods and Results The strains were identified by gyrB gene sequencing and phenotypic tests as B. amyloliquefaciens ssp. plantarum . Their antimicrobial activity was determined by the agar spot and well assay, being inhibitory to a wide range of Gram‐positive and Gram‐negative pathogenic bacteria and fungi. Antimicrobial activity against Bacillus cereus was produced in H. sabdariffa seed‐based medium. PCR results revealed that the isolates have potential for the lipopeptides iturin, fengycin, surfactin, the polyketides difficidin, macrolactin, bacillaene and the dipeptide bacilysin production. Ultra‐high‐performance liquid chromatography‐time of flight mass spectrometry analysis of antimicrobial substance produced in BHI broth allowed identification of iturin, fengycin and surfactin. Conclusions The Bacillus amyloliquefaciens ssp. plantarum exhibited broad‐spectrum antifungal and antibacterial properties. They produced several lipopeptide antibiotics and showed good potential for biological control of Bikalga . Significance and Impact of the Study Pathogenic bacteria often occur in spontaneous food fermentations. This is the first report to identify indigenous B. amyloliquefaciens ssp. plantarum strains as potential protective starter cultures for safeguarding Bikalga .