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Herd prevalence of E nterobacteriaceae producing CTX ‐ M ‐type and CMY ‐2 β ‐lactamases among J apanese dairy farms
Author(s) -
Ohnishi M.,
Okatani A.T.,
Esaki H.,
Harada K.,
Sawada T.,
Murakami M.,
Marumo K.,
Kato Y.,
Sato R.,
Shimura K.,
Hatanaka N.,
Takahashi T.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12211
Subject(s) - enterobacteriaceae , multilocus sequence typing , biology , feces , pulsed field gel electrophoresis , herd , microbiology and biotechnology , cefotaxime , escherichia coli , typing , veterinary medicine , antibiotics , genotype , zoology , medicine , genetics , gene
Aims To determine the herd prevalence of E nterobacteriaceae producing CTX ‐ M ‐type extended‐spectrum β ‐lactamases ( ESBL s) among 381 dairy farms in J apan. Methods and Results Between 2007 and 2009, we screened 897 faecal samples using BTB lactose agar plates containing cefotaxime (2 μg ml −1 ). Positive isolates were tested using ESBL confirmatory tests, PCR and sequencing for CTX ‐ M , A mp C , TEM and SHV . The incidence of E nterobacteriaceae producing CTX ‐ M ‐15 ( n = 7), CTX ‐ M ‐2 ( n = 12), CTX ‐ M ‐14 ( n = 3), CMY ‐2 ( n = 2) or CTX ‐ M ‐15/2/14 and CMY ‐2 ( n = 4) in bovine faeces was 28/897 (3·1%) faecal samples. These genes had spread to E scherichia coli ( n = 23) and three genera of E nterobacteriaceae ( n = 5). Herd prevalence was found to be 20/381 (5·2%) dairy farms. The 23 E . coli isolates showed clonal diversity, as assessed by multilocus sequence typing and pulsed‐field gel electrophoresis. The pandemic E . coli strain ST 131 producing CTX ‐ M ‐15 or CTX ‐ M ‐27 was not detected. Conclusions Three clusters of CTX ‐ M ( CTX ‐ M ‐15, CTX ‐ M ‐2, CTX ‐ M ‐14) had spread among Japanese dairy farms. Significance and Impact of the Study This is the first report on the prevalence of multidrug‐resistant CTX ‐ M ‐15–producing E . coli among J apanese dairy farms.