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Assessment of poly‐ l ‐lysine dendrigrafts for virus concentration in water: use of MS 2 bacteriophage as proof of concept
Author(s) -
Cadiere A.,
Couturaud B.,
Boismard J.,
Cann P.,
Gérard A.,
Mas A.,
Faye C.,
Garrelly L.,
Roig B.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12209
Subject(s) - bacteriophage , lysine , proof of concept , bacteriophage ms2 , virus , bacteria , microbiology and biotechnology , chemistry , biology , biochemistry , food science , amino acid , escherichia coli , virology , computer science , genetics , gene , operating system
Aims Virus detection has often been difficult due to a low concentration in water. In this study, we developed a new procedure based on concentration of virus particles on an innovative support: poly‐ l ‐lysine dendrigrafts ( DGL ), coupled with directed nucleic acid extraction and real‐time PCR quantification. Methods and Results This method was evaluated using the bacteriophage MS 2 as a model virus. This virus exhibited the size and structural properties of human pathogenic enteric viruses and has often been used to assess new supports of concentration. Moreover, this bacteriophage is also a faecal contamination indicator. In this study, many water filtration conditions were tested (volume of water, concentration, etc.), and more than 80% of bacteriophage were recovered after filtration on polymer, in most conditions. We demonstrated that the method was linear (slope = 0·99 ± 0·04 and Y intercept when x = −0·02 ± 0·28), valid (as manipulators, tested concentrations, volumes of sample and batch of polymer did not have any influence on concentration) and sensitive (allowing to concentrate up to 16 600‐fold 1 l of sample and to detect and quantify down to 750 GC l −1 and 7500 GC l −1 , respectively). Conclusions To conclude, this support exhibits high interest to retain viruses and to allow to detect low concentration of virus in water. Significance and Impact of the Study This study gives valuable advance in the methods of concentration and diagnosis of virus in water.