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Isolation and characterization of lactic acid bacteria from Yan‐tsai‐shin (fermented broccoli stems), a traditional fermented food in T aiwan
Author(s) -
Chen Y.s.,
Liou M.s.,
Ji S.h.,
Yu C.r.,
Pan S.f.,
Yanagida F.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12199
Subject(s) - weissella , biology , lactococcus lactis , 16s ribosomal rna , lactococcus , subspecies , lactobacillus , bacteria , fermentation , enterococcus , lactic acid , leuconostoc , food science , microbiology and biotechnology , genetics , zoology
Aims To isolate, characterize and identify lactic acid bacteria ( LAB ) in yan‐tsai‐shin (fermented broccoli stems), a traditional fermented food in T aiwan. Methods and Results A total of 226 LAB were isolated; 111 cultures were isolated from samples collected from seven different markets and 115 from six fresh broccoli samples. These isolates were characterized phenotypically and then initially divided into nine groups (r1 to r9) using restriction fragment length polymorphism analysis and sequencing of 16S ribosomal DNA. Some isolates were further divided into four additional groups by other genetic analysis. The most common bacterial genera in yan‐tsai‐shin and fresh broccoli were W eissella , L actococcus and Lactobacillus . Regional similarities in the LAB, with differences in diversity, were observed in this study. On the basis of phylogenetic analysis of 16S rRNA , rpoA , rpoB and pheS gene sequences, two strains were included in the genera E nterococcus and L actococcus , respectively, and identified as potential novel species or subspecies. In addition, the novel enterococcal strain, and 33 L . lactis subsp. lactis and two W eissella cibaria strains were found to have bacteriocin‐like inhibitory substance (BLIS) producing abilities. Conclusions These results suggest that the LAB play important roles in the fermentation of yan‐tsai‐shin . Significance and Impact of the Study This is the first report describing the distribution and varieties of LAB existing in yan‐tsai‐shin and fresh broccoli. In addition, two potential novel LAB species or subspecies and one potential novel BLIS were also found in this study.