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Hypocrea lixii , novel endophytic fungi producing anticancer agent cajanol, isolated from pigeon pea ( C ajanus cajan [ L .] M illsp.)
Author(s) -
Zhao J.,
Li C.,
Wang W.,
Zhao C.,
Luo M.,
Mu F.,
Fu Y.,
Zu Y.,
Yao M.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12195
Subject(s) - cajanus , plant use of endophytic fungi in defense , biology , hypocrea , botany , horticulture , biochemistry , cellulose , trichoderma reesei , cellulase
Abstract Aims The aim was to isolate, identify and characterize endophytes from pigeon pea ( C ajanus cajan [ L .] M illsp.), as novel producer of cajanol and its in vitro cytotoxicity assay. Methods and Results Isolation, identification and characterization of novel endophytes producing cajanol from the roots of pigeon pea were investigated. The endophytes were identified as H ypocrea lixii by morphological and molecular methods. C ajanol produced by endophytes were quantified by liquid chromatography–tandem mass spectrometry ( LC ‐ MS / MS ). R‐18 produced the highest levels of cajanol (322·4 ± 10·6 μg l −1 or 102·8 ± 6·9 μg g −1 dry weight of mycelium) after incubation for 7 days. The cytotoxicity towards human lung carcinoma cells ( A 549) of fungal cajanol was investigated in vitro . Conclusions First, a novel endophyte H ypocrea lixii , producing anticancer agent cajanol, was isolated from the host pigeon pea ( C ajanus cajan [ L .] M illsp.). Fungal cajanol possessed stronger cytotoxicity activity towards A 549 cells in time‐ and dose‐dependent manners. Significance and Impact of the Study This endophyte is a potential handle for scientific and commercial exploitation, and it could provide a promising alterative approach for large‐scale production of cajanol to satisfy new anticancer drug development.