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Isolation and identification of bacteriocinogenic strain of L actobacillus plantarum with potential beneficial properties from donkey milk
Author(s) -
Murua A.,
Todorov S.D.,
Vieira A.D.S.,
Martinez R.C.R.,
Cencič A.,
Franco B.D.G.M.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12190
Subject(s) - bacteriocin , lactobacillus plantarum , listeria monocytogenes , biopreservation , probiotic , lactic acid , microbiology and biotechnology , lactobacillus , bacteria , enterococcus faecium , biology , food science , donkey , chemistry , fermentation , antimicrobial , antibiotics , ecology , genetics
Aims The goal of this study was to isolate and characterize a lactic acid bacteria ( LAB ) from donkey milk with potential beneficial properties. Methods and Results Lactic acid bacteria were isolated from donkey milk and identified based on physiological, biochemical and molecular methods. The isolate that presented highest bacteriocin potential ( L actobacillus plantarum LP08AD) was evaluated for the production of bacteriocin, including stability in the presence of various enzymes, surfactants, salts, pH and temperatures. Bactericidal effect of bacteriocin LP08AD on L isteria monocytogenes, E nterococcus faecium and L actobacillus curvatus was shown for actively growing and stationary cells. Similar growth and bacteriocin production were observed when strain LP08AD was cultured in MRS broth at 30°C or 37°C. Bacteriocin LP08AD adhered at low levels on the producer cells (200 AU ml −1 ). The presence of plantaricin W gene on the genomic DNA was recorded based on PCR . Good growth for strain LP08AD was recorded in MRS broth with pH from 5·0 to 9·0 and LP08AD grew well in the absence of oxbile or concentration below 0·8%. Lact. plantarum LP08AD was applied to the small intestinal epithelial polarized monolayers of H4, PSIc1 and CLAB and demonstrated low attachment ability on all cell lines studied, with values with a similar behaviour for cells from human and pig origin. Conclusions Bacteriocin‐producing Lact. plantarum LP 08 AD might be useful in the design of novel functional foods with potential probiotic or biopreservation properties. Significance and Impact of the Study To the best of our knowledge, this is the first report on detection and characterization of bacteriocinogenic Lact. plantarum from donkey milk. The strain LP 08 AD shows to have potential beneficial properties, as demonstrated by the use of noncancerogenic cell lines.