Premium
Partial characterization of an exopolysaccharide secreted by a marine bacterium, V ibrio neocaledonicus sp. nov., from N ew C aledonia
Author(s) -
Chalkiadakis E.,
Dufourcq R.,
Schmitt S.,
Brandily C.,
Kervarec N.,
Coatanea D.,
Amir H.,
Loubersac L.,
Chanteau S.,
Guezennec J.,
DupontRouzeyrol M.,
SimonColin C.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12184
Subject(s) - vibrio , microbiology and biotechnology , bacteria , biology , vibrionaceae , marine bacteriophage , genetics
Aims Exopolysaccharides ( EPS ) are industrially valuable molecules with numerous useful properties. This study describes the techniques used for the identification of a novel V ibrio bacterium and preliminary characterization of its EPS . Methods and Results Bioprospection in marine intertidal areas of N ew C aledonia followed by screening for EPS producing brought to selection of the isolate NC 470. Phylogenetic analysis (biochemical tests, gene sequencing and DNA – DNA relatedness) permitted to identify NC 470 as a new member of the V ibrio genus. The EPS was produced in batch fermentation, purified using the ultrafiltration process and analysed by colorimetry, F ourier T ransform I nfrared spectroscopy, gas chromatography, N uclear M agnetic R esonance and HPLC ‐size exclusion chromatography. This EPS exhibits a high N ‐acetyl‐hexosamines and uronic acid content with a low amount of neutral sugar. The molecular mass was 672 × 10 3 Da. These data are relevant for possible technological exploitation. Conclusions We propose the name V ibrio neocaledonicus sp. nov for this isolate NC 470, producing an EPS with an unusual sugar composition. Comparison with other known polymers permitted to select applications for this polymer. Significance and Impact of the Study This study contributes to evaluate the marine biodiversity of N ew C aledonia. It also highlights the biotechnological potential of N ew C aledonia marine bacteria.