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Selection of lactic acid bacteria to promote an efficient silage fermentation capable of inhibiting the activity of A spergillus parasiticus and F usarium gramineraum and mycotoxin production
Author(s) -
Dogi C.A.,
Fochesato A.,
Armando R.,
Pribull B.,
Souza M.M.S.,
da Silva Coelho I.,
Araújo de Melo D.,
Dalcero A.,
Cavaglieri L.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12173
Subject(s) - mycotoxin , silage , fermentation , humanities , aspergillus parasiticus , fusarium , biology , microbiology and biotechnology , food science , art , botany
Abstract Aims To select lactic acid bacteria with potential silage inoculant properties. The bio‐control activity against mycotoxicogenic fungi and the presence of antibiotics resistance gene were also evaluated. Methods and Results Lactobacillus rhamnosus RC007 and L actobacillus plantarum RC009 were selected on the basis of growth rate and efficacy in reducing the pH of maize extract medium; therefore, they were evaluated for their bio‐control ability against F usarium graminearum and A spergillus parasiticus . Studies on lag phase, growth rate and aflatoxin B1 (AFB1) and zearalenone (ZEA) production were carried out in vitro under different regimes of a w (0·95 and 0·99); pH (4 and 6); temperature (25 and 37°C); and oxygen availability (normal and reduced). Lactobacillus rhamnosus RC007 was able to completely inhibit the F. graminearum growth at all assayed conditions, while Lact. plantarum RC009 only did it at pH 4. Both Lactobacillus strains were able to significantly reduce the A. parasiticus growth rate mainly at 0·99 a w . A decrease in ZEA production was observed as result of Lactobacillus strains – F. graminearum interaction; however, the A. parasiticus ‐ Lact. plantarum interaction resulted in an increased AFB1 production. Lactobacillus rhamnosus RC007 proved to have no genes for resistance to the tested antibiotics. Conclusions The ability of Lact. rhamnosus RC007 to rapidly drop the pH and to inhibit fungal growth and mycotoxin production and the absence of antibiotic resistance genes shows the potential of its application as inoculant and bio‐control agent in animal feed. Significance and Impact of the Study This study demonstrated the importance of selecting bacteria for silage inoculants not only for the improvement of silage fermentation but also for their effects on mycotoxicogenic fungi and the resulting mycotoxin production due to the risk that they may involve.